不要重复自己

有时，我们会多次复制粘贴代码以快速处理图像。从长远来看，这种代码重复对代码质量不利，因为如果我们想要更改某些内容，就需要在多个地方进行更改，并可能忘记某些地方。因此，防止重复代码至关重要。这个软件设计原则被称为不要重复自己。

import pyclesperanto_prototype as cle

image = cle.imread("../../data/BBBC007_batch/17P1_POS0013_D_1UL.tif")
labels = cle.voronoi_otsu_labeling(image, spot_sigma=3)
number_of_nuclei = labels.max()
number_of_nuclei

44.0

image = cle.imread("../../data/BBBC007_batch/20P1_POS0005_D_1UL.tif")
labels = cle.voronoi_otsu_labeling(image, spot_sigma=3)
number_of_nuclei = labels.max()
number_of_nuclei

41.0

image = cle.imread("../../data/BBBC007_batch/20P1_POS0007_D_1UL.tif")
labels = cle.voronoi_otsu_labeling(image, spot_sigma=3)
number_of_nuclei = labels.max()
number_of_nuclei

73.0

如果我们现在想看看改变上面的spot_sigma参数如何影响结果，我们需要在三个地方更改这个值。当代码变得更长时，可能会发生我们忘记在某个地方更改它的情况。

使用for循环替代代码重复

防止代码重复的一种方法是使用for循环。

folder = "../../data/BBBC007_batch/"
files = ["17P1_POS0013_D_1UL.tif",
        "20P1_POS0005_D_1UL.tif",
        "20P1_POS0007_D_1UL.tif"]

for file in files:
    image = cle.imread(folder + file)
    labels = cle.voronoi_otsu_labeling(
                    image, 
                    spot_sigma=3)
    number_of_nuclei = labels.max()
    print(file, number_of_nuclei)

17P1_POS0013_D_1UL.tif 44.0
20P1_POS0005_D_1UL.tif 41.0
20P1_POS0007_D_1UL.tif 73.0

使用函数替代代码重复

通过引入所谓的辅助函数，我们可以获得更大的灵活性，这些函数可以_帮助_我们完成一个专门的任务，例如计算细胞核：

def count_nuclei(image, spot_sigma=3):
    labels = cle.voronoi_otsu_labeling(
                    image, 
                    spot_sigma=spot_sigma)
    number_of_nuclei = labels.max()
    
    return number_of_nuclei

count_nuclei(cle.imread(folder + files[0]))

44.0

count_nuclei(cle.imread(folder + files[1]))

41.0

count_nuclei(cle.imread(folder + files[2]))

73.0

count_nuclei(cle.imread(folder + files[2]), spot_sigma=5)

68.0